› 3uj0

X-ray diffraction
3.6Å resolution

Crystal structure of the inositol 1,4,5-trisphosphate receptor with ligand bound form.

Released:
DOI: 10.2210/pdb3uj0/pdb
PDB entry 3uj0 coloured by chain, front view.
PDB entry 3uj0 coloured by chain, side view.
PDB entry 3uj0 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural and functional conservation of key domains in InsP3 and ryanodine receptors.
Seo MD, Velamakanni S, Ishiyama N, Stathopulos PB, Rossi AM, Khan SA, Dale P, Li C, Ames JB, Ikura M, Taylor CW
Nature 483 108-12 (2012)
PMID: 22286060

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol 1,4,5-trisphosphate-gated calcium channel ITPR1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 604 amino acids
Theoretical weight: 67.99 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29994 (Residues: 1-604; Coverage: 22%)
Gene names: Insp3r, Itpr1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand I3P 2 x I3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 189.166Å b: 78.72Å c: 134.107Å
α: 90° β: 124.49° γ: 90°
R-values:
R R work R free
0.26 0.258 0.308
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

34 review citations

Intracellular Ca2+ Sensing: Its Role in Calcium Homeostasis and Signaling.
Bagur et al. (2017)
33 more

15 mentions without citation

Gating machinery of InsP3R channels revealed by electron cryomicroscopy.
Fan et al. (2015)
14 more