› 3ujk

X-ray diffraction
1.9Å resolution

Crystal structure of protein phosphatase ABI2

Released:
DOI: 10.2210/pdb3ujk/pdb
PDB entry 3ujk coloured by chain, front view.
PDB entry 3ujk coloured by chain, side view.
PDB entry 3ujk coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Molecular mimicry regulates ABA signaling by SnRK2 kinases and PP2C phosphatases.
Soon FF, Ng LM, Zhou XE, West GM, Kovach A, Tan MH, Suino-Powell KM, He Y, Xu Y, Chalmers MJ, Brunzelle JS, Zhang H, Yang H, Jiang H, Li J, Yong EL, Cutler S, Zhu JK, Griffin PR, Melcher K, Xu HE
Science 335 85-8 (2012)
PMID: 22116026

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein phosphatase 2C 77 Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 35.78 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O04719 (Residues: 101-423; Coverage: 76%)
Gene names: ABI2, At5g57050, MHM17.19, PP2C77
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3221
Unit cell:
a: 89.666Å b: 89.666Å c: 91.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.195 0.22
Expression system: Escherichia coli

Quick links

Citations

44 review citations

Abiotic Stress Signaling and Responses in Plants.
Zhu JK. (2016)
43 more

3 mentions without citation

Molecular mimicry regulates ABA signaling by SnRK2 kinases and PP2C phosphatases.
Soon et al. (2012)
2 more