› 3upa

X-ray diffraction
1.8Å resolution

A general strategy for the generation of human antibody variable domains with increased aggregation resistance

Released:
DOI: 10.2210/pdb3upa/pdb
PDB entry 3upa coloured by chain, front view.
PDB entry 3upa coloured by chain, side view.
PDB entry 3upa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
General strategy for the generation of human antibody variable domains with increased aggregation resistance.
Dudgeon K, Rouet R, Kokmeijer I, Schofield P, Stolp J, Langley D, Stock D, Christ D
Proc Natl Acad Sci U S A 109 10879-84 (2012)
PMID: 22745168

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin kappa variable 1-39 Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 11.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01597 (Residues: 23-117; Coverage: 100%)
Gene name: IGKV1-39
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P43212
Unit cell:
a: 45.04Å b: 45.04Å c: 173.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.229 0.283
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Therapeutic protein aggregation: mechanisms, design, and control.
Roberts CJ. (2014)
26 more

2 mentions without citation

General strategy for the generation of human antibody variable domains with increased aggregation resistance.
Dudgeon et al. (2012)
1 more