› 3us8

X-ray diffraction
2.25Å resolution

Crystal Structure of an isocitrate dehydrogenase from Sinorhizobium meliloti 1021

Released:
DOI: 10.2210/pdb3us8/pdb
PDB entry 3us8 coloured by chain, front view.
PDB entry 3us8 coloured by chain, side view.
PDB entry 3us8 coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Kumaran D, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, LaFleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187905 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate dehydrogenase [NADP] Chains: A, B
Molecule details ›
Chains: A, B
Length: 427 amino acids
Theoretical weight: 48.44 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92PG6 (Residues: 1-404; Coverage: 100%)
Gene names: SMc00480, icd
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 68.458Å b: 96.208Å c: 146.538Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.18 0.237
Expression system: Escherichia coli

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