› 3uvd

X-ray diffraction
1.85Å resolution

Crystal Structure of the bromodomain of human Transcription activator BRG1 (SMARCA4) in complex with N-Methyl-2-pyrrolidone

Released:
DOI: 10.2210/pdb3uvd/pdb
PDB entry 3uvd coloured by chain, front view.
PDB entry 3uvd coloured by chain, side view.
PDB entry 3uvd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Histone recognition and large-scale structural analysis of the human bromodomain family.
Filippakopoulos P, Picaud S, Mangos M, Keates T, Lambert JP, Barsyte-Lovejoy D, Felletar I, Volkmer R, Müller S, Pawson T, Gingras AC, Arrowsmith CH, Knapp S
Cell 149 214-31 (2012)
PMID: 22464331

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156354 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription activator BRG1 Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51532 (Residues: 1448-1569; Coverage: 7%)
Gene names: BAF190A, BRG1, SMARCA4, SNF2B, SNF2L4
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

1 bound ligand:
Ligand MB3 1 x MB3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 30.2Å b: 29.89Å c: 67.06Å
α: 90° β: 90.45° γ: 90°
R-values:
R R work R free
0.203 0.2 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

245 review citations

Modification of enhancer chromatin: what, how, and why?
Calo et al. (2013)
244 more

1 mention without citation

The molecular basis of selective DNA binding by the BRG1 AT-hook and bromodomain.
Sanchez et al. (2020)