› 3v4r

X-ray diffraction
3.25Å resolution

Crystal structure of a UvrB dimer-DNA complex

Released:
DOI: 10.2210/pdb3v4r/pdb
PDB entry 3v4r coloured by chain, front view.
PDB entry 3v4r coloured by chain, side view.
PDB entry 3v4r coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of the UvrB dimer: insights into the nature and functioning of the UvrAB damage engagement and UvrB-DNA complexes.
Webster MP, Jukes R, Zamfir VS, Kay CW, Bagnéris C, Barrett T
Nucleic Acids Res 40 8743-58 (2012)
PMID: 22753105

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117021 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
UvrABC system protein B Chains: A, B
Molecule details ›
Chains: A, B
Length: 667 amino acids
Theoretical weight: 77.27 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37954 (Residues: 1-661; Coverage: 100%)
Gene names: BSU35170, dinA, uvr, uvrB
Sequence domains:
Structure domains:
DNA: 5 -TACTGTTT-3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 nucleotides
Theoretical weight: 2.41 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 95.94Å b: 100.31Å c: 163.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.219
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Prokaryotic nucleotide excision repair.
Kisker et al. (2013)
2 more

2 mentions without citation

Crystal structure of the UvrB dimer: insights into the nature and functioning of the UvrAB damage engagement and UvrB-DNA complexes.
Webster et al. (2012)
1 more