› 3v4v

X-ray diffraction
3.1Å resolution

crystal structure of a4b7 headpiece complexed with Fab ACT-1 and RO0505376

Released:
DOI: 10.2210/pdb3v4v/pdb
PDB entry 3v4v coloured by chain, front view.
PDB entry 3v4v coloured by chain, side view.
PDB entry 3v4v coloured by chain, top view.
Source organisms:
Primary publication:
Structural specializations of α(4)β(7), an integrin that mediates rolling adhesion.
Yu Y, Zhu J, Mi LZ, Walz T, Sun H, Chen J, Springer TA
J Cell Biol 196 131-46 (2012)
PMID: 22232704

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210552 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Integrin alpha-4 Chains: A, C
Molecule details ›
Chains: A, C
Length: 597 amino acids
Theoretical weight: 65.64 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P13612 (Residues: 34-620; Coverage: 59%)
Gene names: CD49D, ITGA4
Sequence domains:
Structure domains:
Integrin beta-7 Chains: B, D
Molecule details ›
Chains: B, D
Length: 503 amino acids
Theoretical weight: 55.43 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P26010 (Residues: 20-512; Coverage: 63%)
Gene name: ITGB7
Sequence domains:
Structure domains:
MONOCLONAL ANTIBODY Act-1 HEAVY CHAIN Chains: H, M
Molecule details ›
Chains: H, M
Length: 219 amino acids
Theoretical weight: 23.57 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
MONOCLONAL ANTIBODY Act-1 LIGHT CHAIN Chains: L, N
Molecule details ›
Chains: L, N
Length: 217 amino acids
Theoretical weight: 23.9 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
5 bound ligands:
Ligand CA 10 x CA
Ligand NAG 11 x NAG
Ligand TRS 1 x TRS
Ligand MG 2 x MG
Ligand 0DU 2 x 0DU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 127.58Å b: 123.53Å c: 154.16Å
α: 90° β: 112.63° γ: 90°
R-values:
R R work R free
0.198 0.197 0.231
Expression systems:
  • Cricetulus griseus
  • Mus musculus

Quick links

Citations

15 review citations

Assembly and disassembly of cell matrix adhesions.
Wehrle-Haller B. (2012)
14 more

13 mentions without citation

Structural specializations of α(4)β(7), an integrin that mediates rolling adhesion.
Yu et al. (2012)
12 more