› 3v9l

X-ray diffraction
1.5Å resolution

Crystal structure of mouse 1-pyrroline-5-carboxylate dehydrogenase complexed with NAD+

Released:
DOI: 10.2210/pdb3v9l/pdb
PDB entry 3v9l coloured by chain, front view.
PDB entry 3v9l coloured by chain, side view.
PDB entry 3v9l coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The three-dimensional structural basis of type II hyperprolinemia.
Srivastava D, Singh RK, Moxley MA, Henzl MT, Becker DF, Tanner JJ
J Mol Biol 420 176-89 (2012)
PMID: 22516612

Function and Biology Details

Reaction catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183972 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 563 amino acids
Theoretical weight: 61.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8CHT0 (Residues: 21-562; Coverage: 96%)
Gene name: Aldh4a1
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 84.888Å b: 94.053Å c: 132.425Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.158 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Proline mechanisms of stress survival.
Liang et al. (2013)
4 more

3 mentions without citation

The three-dimensional structural basis of type II hyperprolinemia.
Srivastava et al. (2012)
2 more