› 3vac

X-ray diffraction
2.6Å resolution

Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D

Released:
DOI: 10.2210/pdb3vac/pdb
PDB entry 3vac coloured by chain, front view.
PDB entry 3vac coloured by chain, side view.
PDB entry 3vac coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Tight conformational coupling between the domains of the enterotoxigenic Escherichia coli fimbrial adhesin CfaE regulates binding state transition.
Liu Y, Esser L, Interlandi G, Kisiela DI, Tchesnokova V, Thomas WE, Sokurenko E, Xia D, Savarino SJ
J Biol Chem 288 9993-10001 (2013)
PMID: 23393133

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CFA/I fimbrial subunit E Chains: A, B
Molecule details ›
Chains: A, B
Length: 356 amino acids
Theoretical weight: 39.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P25734 (Residues: 23-360; Coverage: 94%)
Gene name: cfaE
Sequence domains: CblD like pilus biogenesis initiator
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 104.066Å b: 126.364Å c: 78.701Å
α: 90° β: 100.49° γ: 90°
R-values:
R R work R free
0.194 0.184 0.234
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Structural and adhesive properties of the long polar fimbriae protein LpfD from adherent-invasive Escherichia coli.
Coppens et al. (2015)
5 more

1 mention without citation

Tight conformational coupling between the domains of the enterotoxigenic Escherichia coli fimbrial adhesin CfaE regulates binding state transition.
Liu et al. (2013)