› 3val

X-ray diffraction
2.5Å resolution

Structure of U2AF65 variant with BrU5C1 DNA

Released:
DOI: 10.2210/pdb3val/pdb
PDB entry 3val coloured by chain, front view.
PDB entry 3val coloured by chain, side view.
PDB entry 3val coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA recognition motifs.
Jenkins JL, Agrawal AA, Gupta A, Green MR, Kielkopf CL
Nucleic Acids Res 41 3859-73 (2013)
PMID: 23376934

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117046 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chains: A, B, D, I
Molecule details ›
Chains: A, B, D, I
Length: 174 amino acids
Theoretical weight: 19.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P26368 (Residues: 148-336; Coverage: 36%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
DNA (5'-D(*C*UP*UP*UP*(BRU)P*UP*U)-3') Chains: E, H, K, P
Molecule details ›
Chains: E, H, K, P
Length: 7 nucleotides
Theoretical weight: 2.06 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand DIO 4 x DIO
Ligand SO4 11 x SO4
1 modified residue:
Ligand BRU 4 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 37.583Å b: 137.081Å c: 83.962Å
α: 90° β: 102.97° γ: 90°
R-values:
R R work R free
0.208 0.208 0.271
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Mechanistic insights into precursor messenger RNA splicing by the spliceosome.
Shi Y. (2017)
6 more

6 mentions without citation

Mapping general anesthetic binding site(s) in human α1β3 γ-aminobutyric acid type A receptors with [³H]TDBzl-etomidate, a photoreactive etomidate analogue.
Chiara et al. (2012)
5 more