› 3vf1

X-ray diffraction
2.47Å resolution

Structure of a calcium-dependent 11R-lipoxygenase suggests a mechanism for Ca-regulation

Released:
DOI: 10.2210/pdb3vf1/pdb
PDB entry 3vf1 coloured by chain, front view.
PDB entry 3vf1 coloured by chain, side view.
PDB entry 3vf1 coloured by chain, top view.
Source organism: Gersemia fruticosa
Primary publication:
Structure of a calcium-dependent 11R-lipoxygenase suggests a mechanism for Ca2+ regulation.
Eek P, Järving R, Järving I, Gilbert NC, Newcomer ME, Samel N
J Biol Chem 287 22377-86 (2012)
PMID: 22573333

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
11R-lipoxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 698 amino acids
Theoretical weight: 79.14 KDa
Source organism: Gersemia fruticosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2N410 (Residues: 1-679; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand FE2 2 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 114.8Å b: 148.71Å c: 117.33Å
α: 90° β: 119.16° γ: 90°
R-values:
R R work R free
0.203 0.202 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

A polycystin-centric view of cyst formation and disease: the polycystins revisited.
Ong et al. (2015)
3 more

4 mentions without citation

The structural basis for specificity in lipoxygenase catalysis.
Newcomer et al. (2015)
3 more