› 3vg9

X-ray diffraction
2.7Å resolution

Crystal structure of human adenosine A2A receptor with an allosteric inverse-agonist antibody at 2.7 A resolution

Released:
DOI: 10.2210/pdb3vg9/pdb
PDB entry 3vg9 coloured by chain, front view.
PDB entry 3vg9 coloured by chain, side view.
PDB entry 3vg9 coloured by chain, top view.
Source organisms:
Primary publication:
G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody.
Hino T, Arakawa T, Iwanari H, Yurugi-Kobayashi T, Ikeda-Suno C, Nakada-Nakura Y, Kusano-Arai O, Weyand S, Shimamura T, Nomura N, Cameron AD, Kobayashi T, Hamakubo T, Iwata S, Murata T
Nature 482 237-40 (2012)
PMID: 22286059

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235084 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Adenosine receptor A2a Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 36.54 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P29274 (Residues: 1-316; Coverage: 77%)
Gene names: ADORA2, ADORA2A
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Structure domains: Rhodopsin 7-helix transmembrane proteins
antibody fab fragment light chain Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 23.53 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
antibody fab fragment heavy chain Chain: C
Molecule details ›
Chain: C
Length: 226 amino acids
Theoretical weight: 24.29 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand ZMA 1 x ZMA
Ligand STE 6 x STE
Ligand LMT 1 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 121.18Å b: 90.15Å c: 113.55Å
α: 90° β: 98.1° γ: 90°
R-values:
R R work R free
0.208 0.206 0.246
Expression system: Komagataella pastoris

Quick links

Citations

47 review citations

Molecular signatures of G-protein-coupled receptors.
Venkatakrishnan et al. (2013)
46 more

38 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
37 more