› › Function and Biology

Crystal structure of the catalytic domain of pyrrolysyl-tRNA synthetase in triclinic crystal form

Source organism: Methanosarcina mazei
Biochemical function: ATP binding
Biological process: tRNA aminoacylation
Cellular component: not assigned

Quick links

EC 6.1.1.26: Pyrrolysine--tRNA(Pyl) ligase

Reaction catalysed:
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl)
Systematic name:
L-pyrrolysine:tRNA(Pyl) ligase (AMP-forming)
Alternative Name(s):
  • PylRS
  • PylS
  • Pyrrolysyl-tRNA synthetase

GO terms

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Sequence family

Pfam Protein family (Pfam)
PF01409
Domain description: tRNA synthetases class II core domain (F)
Occurring in:
  1. Pyrrolysine--tRNA ligase
The deposited structure of PDB entry 3vqx contains 4 copies of Pfam domain PF01409 (tRNA synthetases class II core domain (F)) in Pyrrolysine--tRNA ligase. Showing 1 copy in chain D.

InterPro InterPro annotations
IPR045864
Domain description: Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL)
Occurring in:
  1. Pyrrolysine--tRNA ligase
IPR006195
Domain description: Aminoacyl-tRNA synthetase, class II
Occurring in:
  1. Pyrrolysine--tRNA ligase
IPR002319
Domain description: Phenylalanyl-tRNA synthetase
Occurring in:
  1. Pyrrolysine--tRNA ligase
IPR023877
Domain description: Pyrrolysyl-tRNA ligase, C-terminal
Occurring in:
  1. Pyrrolysine--tRNA ligase
IPR014729
Domain description: Rossmann-like alpha/beta/alpha sandwich fold
Occurring in:
  1. Pyrrolysine--tRNA ligase

Structure domains

CATH CATH domains
3.30.930.10
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: BirA Bifunctional Protein; domain 2
Homology: Bira Bifunctional Protein; Domain 2
Occurring in:
  1. Pyrrolysine--tRNA ligase
The deposited structure of PDB entry 3vqx contains 4 copies of CATH domain 3.30.930.10 (BirA Bifunctional Protein; domain 2) in Pyrrolysine--tRNA ligase. Showing 1 copy in chain D.
1.10.287.540
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Helix Hairpins
Homology: Helix hairpin bin
Occurring in:
  1. Pyrrolysine--tRNA ligase
The deposited structure of PDB entry 3vqx contains 4 copies of CATH domain 1.10.287.540 (Helix Hairpins) in Pyrrolysine--tRNA ligase. Showing 1 copy in chain D.

Quick links