› 3vtn

X-ray diffraction
1.75Å resolution

The crystal structure of the C-terminal domain of Mu phage central spike - Pt derivative for MAD

Released:
DOI: 10.2210/pdb3vtn/pdb
PDB entry 3vtn coloured by chain, front view.
PDB entry 3vtn coloured by chain, side view.
PDB entry 3vtn coloured by chain, top view.
Source organism: Escherichia virus Mu
Primary publication:
Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion.
Harada K, Yamashita E, Nakagawa A, Miyafusa T, Tsumoto K, Ueno T, Toyama Y, Takeda S
Biochim Biophys Acta 1834 284-91 (2013)
PMID: 22922659

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Baseplate puncturing device gp45 Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 10.91 KDa
Source organism: Escherichia virus Mu
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9T1V4 (Residues: 100-197; Coverage: 50%)
Gene name: Mup45
Sequence domains: Phage spike trimer
Structure domains: N-terminal domain of TfIIb

Ligands and Environments

2 bound ligands:
Ligand PT 1 x PT
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P321
Unit cell:
a: 47.171Å b: 47.171Å c: 72.114Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.274 0.273 0.304
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Artificial bio-nanomachines based on protein needles derived from bacteriophage T4.
Inaba et al. (2018)
11 other articles

1 mention without citation

Combination Therapy of Phage vB_KpnM_P-KP2 and Gentamicin Combats Acute Pneumonia Caused by K47 Serotype Klebsiella pneumoniae.
Wang et al. (2021)