› 3vyy

X-ray diffraction
2.9Å resolution

Structural insights into RISC assembly facilitated by dsRNA binding domains of human RNA helicase A (DHX9)

Released:
DOI: 10.2210/pdb3vyy/pdb
PDB entry 3vyy coloured by chain, front view.
PDB entry 3vyy coloured by chain, side view.
PDB entry 3vyy coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into RISC assembly facilitated by dsRNA-binding domains of human RNA helicase A (DHX9).
Fu Q, Yuan YA
Nucleic Acids Res 41 3457-70 (2013)
PMID: 23361462

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-170611 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
ATP-dependent RNA helicase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 91 amino acids
Theoretical weight: 10.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08211 (Residues: 1-91; Coverage: 7%)
Gene names: DDX9, DHX9, LKP, NDH2
Sequence domains: Double-stranded RNA binding motif
Structure domains: Double Stranded RNA Binding Domain
RNA (5'-R(*GP*CP*GP*CP*GP*CP*GP*CP*GP*C)-3') Chains: C, D, E, F, G
Molecule details ›
Chains: C, D, E, F, G
Length: 10 nucleotides
Theoretical weight: 3.21 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 52.252Å b: 78.529Å c: 112.242Å
α: 90° β: 98.81° γ: 90°
R-values:
R R work R free
0.221 0.219 0.262
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

The biology of DHX9 and its potential as a therapeutic target.
Lee et al. (2016)
4 more

10 mentions without citation

'Black sheep' that don't leave the double-stranded RNA-binding domain fold.
Gleghorn et al. (2014)
9 more