› 3w13

X-ray diffraction
4.3Å resolution

Insulin receptor ectodomain construct comprising domains L1-CR in complex with high-affinity insulin analogue [D-PRO-B26]-DTI-NH2, alphact peptide(693-719) and FAB 83-7

Released:
DOI: 10.2210/pdb3w13/pdb
PDB entry 3w13 coloured by chain, front view.
PDB entry 3w13 coloured by chain, side view.
PDB entry 3w13 coloured by chain, top view.
Source organisms:
Primary publication:
How insulin engages its primary binding site on the insulin receptor.
Menting JG, Whittaker J, Margetts MB, Whittaker LJ, Kong GK, Smith BJ, Watson CJ, Záková L, Kletvíková E, Jiráček J, Chan SJ, Steiner DF, Dodson GG, Brzozowski AM, Weiss MA, Ward CW, Lawrence MC
Nature 493 241-5 (2013)
PMID: 23302862

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235013 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (8 distinct):
Insulin receptor subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 310 amino acids
Theoretical weight: 35.5 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06213 (Residues: 28-337; Coverage: 23%)
Gene name: INSR
Sequence domains:
monoclonal antibody fab 83-7 fragment - heavy chain Chain: C
Molecule details ›
Chain: C
Length: 116 amino acids
Theoretical weight: 12.71 KDa
Source organism: Mus musculus
Expression system: Mus musculus
monoclonal antibody fab 83-7 fragment - light chain Chain: D
Molecule details ›
Chain: D
Length: 114 amino acids
Theoretical weight: 12.68 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 26 amino acids
Theoretical weight: 2.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-49; Coverage: 29%)
Gene name: INS
Insulin receptor subunit alpha Chain: F
Molecule details ›
Chain: F
Length: 23 amino acids
Theoretical weight: 2.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P06213 (Residues: 724-746; Coverage: 2%)
Gene name: INSR

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
1 modified residue:
Ligand PR9 1 x PR9

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P23
Unit cell:
a: 169.228Å b: 169.228Å c: 169.228Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.291 0.289 0.335
Expression systems:
  • Cricetulus griseus
  • Mus musculus
  • Not provided

Quick links

Citations

59 review citations

Insulin Receptor Isoforms in Physiology and Disease: An Updated View.
Belfiore et al. (2017)
58 more

2 mentions without citation

How insulin engages its primary binding site on the insulin receptor.
Menting et al. (2013)
1 more