› 3w5k

X-ray diffraction
2.6Å resolution

Crystal structure of Snail1 and importin beta complex

Released:
DOI: 10.2210/pdb3w5k/pdb
PDB entry 3w5k coloured by chain, front view.
PDB entry 3w5k coloured by chain, side view.
PDB entry 3w5k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the selective nuclear import of the C2H2 zinc-finger protein Snail by importin β.
Choi S, Yamashita E, Yasuhara N, Song J, Son SY, Won YH, Hong HR, Shin YS, Sekimoto T, Park IY, Yoneda Y, Lee SJ
Acta Crystallogr D Biol Crystallogr 70 1050-60 (2014)
PMID: 24699649

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132074 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit beta-1 Chain: A
Molecule details ›
Chain: A
Length: 876 amino acids
Theoretical weight: 97.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14974 (Residues: 1-876; Coverage: 100%)
Gene names: KPNB1, NTF97
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Zinc finger protein SNAI1 Chain: B
Molecule details ›
Chain: B
Length: 264 amino acids
Theoretical weight: 29.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95863 (Residues: 1-264; Coverage: 100%)
Gene names: SNAH, SNAI1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU, PAL/PLS BEAMLINE 6C1
Spacegroup: C2
Unit cell:
a: 228.21Å b: 77.528Å c: 72.019Å
α: 90° β: 100.96° γ: 90°
R-values:
R R work R free
0.217 0.215 0.264
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structural Biology and Regulation of Protein Import into the Nucleus.
Christie et al. (2016)
2 more

7 mentions without citation

Karyopherin-mediated nucleocytoplasmic transport.
Wing et al. (2022)
6 more