› 3w7f

X-ray diffraction
2.25Å resolution

Crystal structure of the C(30) carotenoid dehydrosqualene synthase from staphylococcus aureus complexed with farnesyl thiopyrophosphate

Released:
DOI: 10.2210/pdb3w7f/pdb
PDB entry 3w7f coloured by chain, front view.
PDB entry 3w7f coloured by chain, side view.
PDB entry 3w7f coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
A cholesterol biosynthesis inhibitor blocks Staphylococcus aureus virulence.
Liu CI, Liu GY, Song Y, Yin F, Hensler ME, Jeng WY, Nizet V, Wang AH, Oldfield E
Science 319 1391-4 (2008)
PMID: 18276850

Function and Biology Details

Reaction catalysed: 
(1a) 2 (2E,6E)-farnesyl diphosphate = diphosphate + presqualene diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108994 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4,4'-diapophytoene synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 293 amino acids
Theoretical weight: 34.79 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: A9JQL9 (Residues: 1-287; Coverage: 100%)
Gene name: crtM
Sequence domains: Squalene/phytoene synthase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

2 bound ligands:
Ligand FPS 4 x FPS
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P3121
Unit cell:
a: 80.06Å b: 80.06Å c: 183.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.265
Expression system: Escherichia coli

Quick links

Citations

68 review citations

Sticking together: building a biofilm the Bacillus subtilis way.
Vlamakis et al. (2013)
67 more

6 mentions without citation

Candida albicans quorum-sensing molecule farnesol modulates staphyloxanthin production and activates the thiol-based oxidative-stress response in Staphylococcus aureus.
Vila et al. (2019)
5 more