› 3w8r

X-ray diffraction
2.5Å resolution

Mutant structure of Thermus thermophilus HB8 uridine-cytidine kinase

Released:
DOI: 10.2210/pdb3w8r/pdb
PDB entry 3w8r coloured by chain, front view.
PDB entry 3w8r coloured by chain, side view.
PDB entry 3w8r coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Indispensable residue for uridine binding in the uridine-cytidine kinase family.
Tomoike F, Nakagawa N, Fukui K, Yano T, Kuramitsu S, Masui R
Biochem Biophys Rep 11 93-98 (2017)
PMID: 28955773

Function and Biology Details

Reaction catalysed: 
ATP + cytidine = ADP + CMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178105 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.68 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SKR5 (Residues: 1-211; Coverage: 100%)
Gene names: TTHA0578, udk
Sequence domains: Phosphoribulokinase / Uridine kinase family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand CTN 2 x CTN
Ligand ACP 2 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P21212
Unit cell:
a: 68.3Å b: 124.1Å c: 60.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.283
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Dermatophyte Resistance to Antifungal Drugs: Mechanisms and Prospectus.
Martinez-Rossi et al. (2018)
1 more

3 mentions without citation

Indispensable residue for uridine binding in the uridine-cytidine kinase family.
Tomoike et al. (2017)
2 more