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Fitting result in the O-layer of the subnanometer structure of the bacterial pKM101 type IV secretion system core complex digested with elastase

Electron Microscopy
8.5Å resolution
PDB entry 3zbi coloured by chain, front view.
PDB entry 3zbi coloured by chain, side view.
PDB entry 3zbi coloured by chain, top view.
Source organism: Escherichia coli BL21
Assembly composition:
hetero 42-mer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 42-mer
PDBe Complex ID: PDB-CPX-175133

Macromolecules

TraF protein
Chains: A, D, G, J, M, P, S, V, Y, b, e, h, k, n
Length: 216 amino acids
Theoretical weight: 22.96 KDa
Source organism: Escherichia coli BL21
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q46705 (Residues: 171-386; Coverage: 56%)
Gene name: traF
Pfam: Bacterial conjugation TrbI-like protein
InterPro:
TraF protein in PDB entry 3zbi, assembly 1, front view.
TraF protein in PDB entry 3zbi, assembly 1, side view.
TraF protein in PDB entry 3zbi, assembly 1, top view.
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TraO protein
Chains: B, E, H, K, N, Q, T, W, Z, c, f, i, l, o
Length: 130 amino acids
Theoretical weight: 14.68 KDa
Source organism: Escherichia coli BL21
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q46704 (Residues: 161-290; Coverage: 48%)
Gene name: traO
Pfam: Conjugal transfer protein
InterPro:
TraO protein in PDB entry 3zbi, assembly 1, front view.
TraO protein in PDB entry 3zbi, assembly 1, side view.
TraO protein in PDB entry 3zbi, assembly 1, top view.
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TraN protein
Chains: C, F, I, L, O, R, U, X, a, d, g, j, m, p
Length: 48 amino acids
Theoretical weight: 5.14 KDa
Source organism: Escherichia coli BL21
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q46702 (Residues: 1-48; Coverage: 100%)
Gene name: traN
Pfam: P-type Type IV secretion system, TraN
TraN protein in PDB entry 3zbi, assembly 1, front view.
TraN protein in PDB entry 3zbi, assembly 1, side view.
TraN protein in PDB entry 3zbi, assembly 1, top view.
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