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Cryo-EM structure of the F420-reducing NiFe-hydrogenase from a methanogenic archaeon with bound substrate

Electron Microscopy
4Å resolution
PDB entry 3zfs coloured by chain, front view.
PDB entry 3zfs coloured by chain, side view.
PDB entry 3zfs coloured by chain, top view.
Source organism: Methanothermobacter marburgensis
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero trimer
PDBe Complex ID: PDB-CPX-113016

Macromolecules

F420-reducing hydrogenase, subunit alpha
Chain: A
Length: 405 amino acids
Theoretical weight: 44.87 KDa
Source organism: Methanothermobacter marburgensis
UniProt:
  • Canonical: D9PYF9 (Residues: 1-405; Coverage: 100%)
Gene names: MTBMA_c16860, frhA
Pfam: Nickel-dependent hydrogenase
InterPro:
F420-reducing hydrogenase, subunit alpha in PDB entry 3zfs, assembly 1, front view.
F420-reducing hydrogenase, subunit alpha in PDB entry 3zfs, assembly 1, side view.
F420-reducing hydrogenase, subunit alpha in PDB entry 3zfs, assembly 1, top view.
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4Fe-4S ferredoxin-type domain-containing protein
Chain: B
Length: 275 amino acids
Theoretical weight: 30.27 KDa
Source organism: Methanothermobacter marburgensis
UniProt:
  • Canonical: D9PYF7 (Residues: 1-275; Coverage: 100%)
Gene names: MTBMA_c16840, frhG
Pfam:
InterPro:
4Fe-4S ferredoxin-type domain-containing protein in PDB entry 3zfs, assembly 1, front view.
4Fe-4S ferredoxin-type domain-containing protein in PDB entry 3zfs, assembly 1, side view.
4Fe-4S ferredoxin-type domain-containing protein in PDB entry 3zfs, assembly 1, top view.
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F420-reducing hydrogenase, subunit beta
Chain: C
Length: 281 amino acids
Theoretical weight: 30.78 KDa
Source organism: Methanothermobacter marburgensis
UniProt:
  • Canonical: D9PYF6 (Residues: 1-281; Coverage: 100%)
Gene names: MTBMA_c16830, frhB
Pfam:
InterPro:
F420-reducing hydrogenase, subunit beta in PDB entry 3zfs, assembly 1, front view.
F420-reducing hydrogenase, subunit beta in PDB entry 3zfs, assembly 1, side view.
F420-reducing hydrogenase, subunit beta in PDB entry 3zfs, assembly 1, top view.
Molecule details ›

Search similar proteins

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