› 3zfx

X-ray diffraction
2.5Å resolution

Crystal structure of EphB1

Released:
DOI: 10.2210/pdb3zfx/pdb
PDB entry 3zfx coloured by chain, front view.
PDB entry 3zfx coloured by chain, side view.
PDB entry 3zfx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Completing the structural family portrait of the human EphB tyrosine kinase domains.
Overman RC, Debreczeni JE, Truman CM, McAlister MS, Attwood TK
Protein Sci 23 627-38 (2014)
PMID: 24677421

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157092 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ephrin type-B receptor 1 Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 298 amino acids
Theoretical weight: 33.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54762 (Residues: 602-896; Coverage: 31%)
Gene names: ELK, EPHB1, EPHT2, HEK6, NET
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14
Spacegroup: P32
Unit cell:
a: 195.98Å b: 195.98Å c: 60.242Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Factors involved in heterologous expression of proteins in E. coli host.
Pouresmaeil et al. (2023)
8 other articles

4 mentions without citation

A Chemical-Genetic Approach to Generate Selective Covalent Inhibitors of Protein Kinases.
Kung et al. (2017)
3 more