› 3zgb

X-ray diffraction
2.71Å resolution

Greater efficiency of photosynthetic carbon fixation due to single amino acid substitution

Released:
DOI: 10.2210/pdb3zgb/pdb
PDB entry 3zgb coloured by chain, front view.
PDB entry 3zgb coloured by chain, side view.
PDB entry 3zgb coloured by chain, top view.
Source organism: Flaveria pringlei
Primary publication:
Greater efficiency of photosynthetic carbon fixation due to single amino-acid substitution.
Paulus JK, Schlieper D, Groth G
Nat Commun 4 1518 (2013)
PMID: 23443546

Function and Biology Details

Reaction catalysed: 
Phosphate + oxaloacetate = H(2)O + phosphoenolpyruvate + HCO(3)(-)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169608 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 972 amino acids
Theoretical weight: 111.47 KDa
Source organism: Flaveria pringlei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01647 (Residues: 6-967; Coverage: 99%)
Gene name: PPCA1
Sequence domains: Phosphoenolpyruvate carboxylase

Ligands and Environments

3 bound ligands:
Ligand ASP 2 x ASP
Ligand EDO 2 x EDO
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P21212
Unit cell:
a: 165.549Å b: 121.7Å c: 132.031Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.23
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Using Biotechnology-Led Approaches to Uplift Cereal and Food Legume Yields in Dryland Environments.
Dwivedi et al. (2018)
1 more

6 mentions without citation

Greater efficiency of photosynthetic carbon fixation due to single amino-acid substitution.
Paulus et al. (2013)
5 more