› 3zig

X-ray diffraction
2.5Å resolution

SepF-like protein from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb3zig/pdb
PDB entry 3zig coloured by chain, front view.
PDB entry 3zig coloured by chain, side view.
PDB entry 3zig coloured by chain, top view.
Source organism: Pyrococcus furiosus COM1
Primary publication:
Structural and genetic analyses reveal the protein SepF as a new membrane anchor for the Z ring.
Duman R, Ishikawa S, Celik I, Strahl H, Ogasawara N, Troc P, Löwe J, Hamoen LW
Proc Natl Acad Sci U S A 110 E4601-10 (2013)
PMID: 24218584

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division protein SepF Chains: A, B
Molecule details ›
Chains: A, B
Length: 86 amino acids
Theoretical weight: 9.5 KDa
Source organism: Pyrococcus furiosus COM1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I6V3Q6 (Residues: 46-131; Coverage: 66%)
Gene name: PFC_09450
Sequence domains: Cell division protein SepF
Structure domains: SepF-like protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6522
Unit cell:
a: 59.327Å b: 59.327Å c: 177.484Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.219 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Splitsville: structural and functional insights into the dynamic bacterial Z ring.
Haeusser et al. (2016)
20 more

2 mentions without citation

Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum.
Sogues et al. (2020)
1 more