› 3ziu

X-ray diffraction
2.07Å resolution

Crystal structure of Mycoplasma mobile Leucyl-tRNA Synthetase with Leu-AMS in the active site

Released:
DOI: 10.2210/pdb3ziu/pdb
PDB entry 3ziu coloured by chain, front view.
PDB entry 3ziu coloured by chain, side view.
PDB entry 3ziu coloured by chain, top view.
Source organism: Mesomycoplasma mobile
Primary publication:
Leucyl-tRNA synthetase editing domain functions as a molecular rheostat to control codon ambiguity in Mycoplasma pathogens.
Li L, Palencia A, Lukk T, Li Z, Luthey-Schulten ZA, Cusack S, Martinis SA, Boniecki MT
Proc Natl Acad Sci U S A 110 3817-22 (2013)
PMID: 23431144

Function and Biology Details

Reaction catalysed: 
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179833 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
leucine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 637 amino acids
Theoretical weight: 75.13 KDa
Source organism: Mesomycoplasma mobile
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6KHA5 (Residues: 1-630; Coverage: 100%)
Gene names: MMOB5390, leuS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand LSS 2 x LSS
Ligand GOL 10 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: R32
Unit cell:
a: 202.851Å b: 202.851Å c: 213.922Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Protein mistranslation: friend or foe?
Ribas de Pouplana et al. (2014)
4 more

2 mentions without citation

Leucyl-tRNA synthetase editing domain functions as a molecular rheostat to control codon ambiguity in Mycoplasma pathogens.
Li et al. (2013)
1 more