› 3zkc

X-ray diffraction
3Å resolution

Crystal structure of the master regulator for biofilm formation SinR in complex with DNA.

Released:
DOI: 10.2210/pdb3zkc/pdb
PDB entry 3zkc coloured by chain, front view.
PDB entry 3zkc coloured by chain, side view.
PDB entry 3zkc coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Molecular basis of the activity of SinR protein, the master regulator of biofilm formation in Bacillus subtilis.
Newman JA, Rodrigues C, Lewis RJ
J Biol Chem 288 10766-78 (2013)
PMID: 23430750

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117118 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH-type transcriptional regulator SinR Chains: A, B
Molecule details ›
Chains: A, B
Length: 111 amino acids
Theoretical weight: 13.01 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06533 (Residues: 1-111; Coverage: 100%)
Gene names: BSU24610, flaD, sin, sinR
Sequence domains:
Structure domains: lambda repressor-like DNA-binding domains
5'-D(*AP*AP*AP*GP*TP*TP*CP*TP*CP*TP*TP*TP*AP*GP *AP*GP*AP*AP*CP*AP*AP)-3' Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Not provided
5'-D(*AP*TP*TP*GP*TP*TP*CP*TP*CP*TP*AP*AP*AP*GP *AP*GP*AP*AP*CP*TP*TP)-3' Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21212
Unit cell:
a: 65.364Å b: 79.739Å c: 67.939Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.242 0.264
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Proteinaceous determinants of surface colonization in bacteria: bacterial adhesion and biofilm formation from a protein secretion perspective.
Chagnot et al. (2013)
5 more

9 mentions without citation

Tetrameric c-di-GMP mediates effective transcription factor dimerization to control Streptomyces development.
Tschowri et al. (2014)
8 more