› 3zpl

X-ray diffraction
2.8Å resolution

Crystal structure of Sco3205, a MarR family transcriptional regulator from Streptomyces coelicolor, in complex with DNA

Released:
DOI: 10.2210/pdb3zpl/pdb
PDB entry 3zpl coloured by chain, front view.
PDB entry 3zpl coloured by chain, side view.
PDB entry 3zpl coloured by chain, top view.
Source organisms:
Primary publication:
Investigation of DNA sequence recognition by a streptomycete MarR family transcriptional regulator through surface plasmon resonance and X-ray crystallography.
Stevenson CE, Assaad A, Chandra G, Le TB, Greive SJ, Bibb MJ, Lawson DM
Nucleic Acids Res 41 7009-22 (2013)
PMID: 23748564

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117121 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH marR-type domain-containing protein Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 177 amino acids
Theoretical weight: 20.69 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KYU1 (Residues: 1-163; Coverage: 100%)
Gene names: SCE22.22, SCO3205
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*AP*AP*AP*GP*AP*TP*TP*GP*AP*GP*AP*TP*CP*TP *CP*AP*AP*TP*CP*TP*TP*DT)-3' Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 22 nucleotides
Theoretical weight: 6.75 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P65
Unit cell:
a: 70.8Å b: 70.8Å c: 557.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.176 0.196
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

The evolution of MarR family transcription factors as counter-silencers in regulatory networks.
Will et al. (2020)
28 other articles

5 mentions without citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
4 more