› 3zqs

X-ray diffraction
2Å resolution

Human FANCL central domain

Released:
DOI: 10.2210/pdb3zqs/pdb
PDB entry 3zqs coloured by chain, front view.
PDB entry 3zqs coloured by chain, side view.
PDB entry 3zqs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of human FANCL, the E3 ligase in the Fanconi anemia pathway.
Hodson C, Cole AR, Lewis LP, Miles JA, Purkiss A, Walden H
J Biol Chem 286 32628-37 (2011)
PMID: 21775430

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase FANCL Chains: A, B
Molecule details ›
Chains: A, B
Length: 186 amino acids
Theoretical weight: 21.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NW38 (Residues: 109-294; Coverage: 50%)
Gene names: FANCL, PHF9
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PRO 2 x PRO
Ligand P6G 10 x P6G
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 147.6Å b: 102.52Å c: 65.76Å
α: 90° β: 94.09° γ: 90°
R-values:
R R work R free
0.184 0.183 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The Fanconi anemia DNA repair pathway: structural and functional insights into a complex disorder.
Walden et al. (2014)
9 more

9 mentions without citation

Specificity and disease in the ubiquitin system.
Chaugule et al. (2016)
8 more