› 3zr6

X-ray diffraction
2.44Å resolution

STRUCTURE OF GALACTOCEREBROSIDASE FROM MOUSE IN COMPLEX WITH GALACTOSE

Released:
DOI: 10.2210/pdb3zr6/pdb
PDB entry 3zr6 coloured by chain, front view.
PDB entry 3zr6 coloured by chain, side view.
PDB entry 3zr6 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Insights into Krabbe disease from structures of galactocerebrosidase.
Deane JE, Graham SC, Kim NN, Stein PE, McNair R, Cachón-González MB, Cox TM, Read RJ
Proc Natl Acad Sci U S A 108 15169-73 (2011)
PMID: 21876145

Function and Biology Details

Reaction catalysed: 
D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Galactocerebrosidase Chain: A
Molecule details ›
Chain: A
Length: 656 amino acids
Theoretical weight: 74.75 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P54818 (Residues: 40-684; Coverage: 100%)
Gene name: Galc
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
3 bound ligands:
Ligand NAG 2 x NAG
Ligand GAL 1 x GAL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: R32
Unit cell:
a: 249.548Å b: 249.548Å c: 77.456Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.178 0.223
Expression system: Homo sapiens

Quick links

Citations

19 review citations

On the nature of mycobacteriophage diversity and host preference.
Jacobs-Sera et al. (2012)
18 more

4 mentions without citation

New therapeutic approaches for Krabbe disease: The potential of pharmacological chaperones.
Spratley et al. (2016)
3 more