› 3zsf

X-ray diffraction
2.32Å resolution

Crystal structure of the L-cystine solute receptor of Neisseria gonorrhoeae in the unliganded open conformation

Released:
DOI: 10.2210/pdb3zsf/pdb
PDB entry 3zsf coloured by chain, front view.
PDB entry 3zsf coloured by chain, side view.
PDB entry 3zsf coloured by chain, top view.
Source organism: Neisseria gonorrhoeae
Primary publication:
Crystal structures of two solute receptors for L-cystine and L-cysteine, respectively, of the human pathogen Neisseria gonorrhoeae.
Bulut H, Moniot S, Licht A, Scheffel F, Gathmann S, Saenger W, Schneider E
J Mol Biol 415 560-72 (2012)
PMID: 22138345

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Solute-binding protein family 3/N-terminal domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 283 amino acids
Theoretical weight: 30.07 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5F9M1 (Residues: 16-274; Coverage: 100%)
Gene name: NGO_0372
Sequence domains: Bacterial extracellular solute-binding proteins, family 3
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 74.09Å b: 99.83Å c: 128.83Å
α: 90° β: 91.5° γ: 90°
R-values:
R R work R free
0.22 0.217 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Mechanism of Action of ABC Importers: Conservation, Divergence, and Physiological Adaptations.
Lewinson et al. (2017)
3 more