› 3zuf

X-ray diffraction
2.2Å resolution

Padron off (non-fluorescent) Btrans

Released:
DOI: 10.2210/pdb3zuf/pdb
PDB entry 3zuf coloured by chain, front view.
PDB entry 3zuf coloured by chain, side view.
PDB entry 3zuf coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
Low-temperature chromophore isomerization reveals the photoswitching mechanism of the fluorescent protein Padron.
Faro AR, Carpentier P, Jonasson G, Pompidor G, Arcizet D, Demachy I, Bourgeois D
J Am Chem Soc 133 16362-5 (2011)
PMID: 21923132

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 215 amino acids
Theoretical weight: 24.65 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5TLG6 (Residues: 2-218; Coverage: 97%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYC 6 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21212
Unit cell:
a: 109.32Å b: 182Å c: 72.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.24
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Fluorescent proteins for live-cell imaging with super-resolution.
Nienhaus et al. (2014)
9 more