› 3zuj

X-ray diffraction
2.34Å resolution

Padron on (fluorescent) ABcis

Released:
DOI: 10.2210/pdb3zuj/pdb
PDB entry 3zuj coloured by chain, front view.
PDB entry 3zuj coloured by chain, side view.
PDB entry 3zuj coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
Low-temperature chromophore isomerization reveals the photoswitching mechanism of the fluorescent protein Padron.
Faro AR, Carpentier P, Jonasson G, Pompidor G, Arcizet D, Demachy I, Bourgeois D
J Am Chem Soc 133 16362-5 (2011)
PMID: 21923132

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 214 amino acids
Theoretical weight: 24.52 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5TLG6 (Residues: 2-217; Coverage: 96%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYC 6 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 109.58Å b: 181.56Å c: 72.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.25
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Fluorescent proteins for live-cell imaging with super-resolution.
Nienhaus et al. (2014)
9 more

1 mention without citation

The Positive Switching Fluorescent Protein Padron2 Enables Live-Cell Reversible Saturable Optical Linear Fluorescence Transitions (RESOLFT) Nanoscopy without Sequential Illumination Steps.
Konen et al. (2021)