› 3zut

X-ray diffraction
2.5Å resolution

The structure of OST1 (D160A) kinase

Released:
DOI: 10.2210/pdb3zut/pdb
PDB entry 3zut coloured by chain, front view.
PDB entry 3zut coloured by chain, side view.
PDB entry 3zut coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The structure of Arabidopsis thaliana OST1 provides insights into the kinase regulation mechanism in response to osmotic stress.
Yunta C, Martínez-Ripoll M, Zhu JK, Albert A
J Mol Biol 414 135-44 (2011)
PMID: 21983340

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188173 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase SRK2E Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 41.05 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q940H6 (Residues: 1-362; Coverage: 100%)
Gene names: At4g33950, F17I5.140, OST1, SNRK2.6, SRK2E
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P2221
Unit cell:
a: 77.273Å b: 99.195Å c: 107.832Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.251
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Type 2C protein phosphatases in plants.
Fuchs et al. (2013)
8 more

2 mentions without citation

The structure of Arabidopsis thaliana OST1 provides insights into the kinase regulation mechanism in response to osmotic stress.
Yunta et al. (2011)
1 more