› 3zxw

X-ray diffraction
2.1Å resolution

STRUCTURE OF ACTIVATED RUBISCO FROM THERMOSYNECHOCOCCUS ELONGATUS COMPLEXED WITH 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE

Released:
DOI: 10.2210/pdb3zxw/pdb
PDB entry 3zxw coloured by chain, front view.
PDB entry 3zxw coloured by chain, side view.
PDB entry 3zxw coloured by chain, top view.
Source organism: Thermosynechococcus vestitus BP-1
Entry authors: Terlecka B, Wilhelmi V, Bialek W, Gubernator B, Szczepaniak A, Hofmann E

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184071 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribulose bisphosphate carboxylase large chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 475 amino acids
Theoretical weight: 53.13 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: Q8DIS5 (Residues: 1-475; Coverage: 100%)
Gene names: cbbL, rbcL, tll1506
Sequence domains:
Structure domains:
Ribulose bisphosphate carboxylase small subunit Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 118 amino acids
Theoretical weight: 14 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: Q8DIS7 (Residues: 1-118; Coverage: 100%)
Gene names: cbbS, rbcS, tll1504
Sequence domains: Ribulose bisphosphate carboxylase, small chain
Structure domains: Ribulose bisphosphate carboxylase, small subunit

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand CAP 4 x CAP
Ligand GOL 11 x GOL
1 modified residue:
Ligand KCX 4 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P43212
Unit cell:
a: 111.5Å b: 111.5Å c: 397Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.214
Expression system: Escherichia coli DH5[alpha]

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