› 404d

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF THE RNA/DNA HYBRID R(GAAGAGAAGC). D(GCTTCTCTTC)

Released:
DOI: 10.2210/pdb404d/pdb
PDB entry 404d coloured by chain, front view.
PDB entry 404d coloured by chain, side view.
PDB entry 404d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
The crystal structure of the RNA/DNA hybrid r(GAAGAGAAGC). d(GCTTCTCTTC) shows significant differences to that found in solution.
Conn GL, Brown T, Leonard GA
Nucleic Acids Res 27 555-61 (1999)
PMID: 9862980

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-117129 (preferred)
Entry contents:
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
5'-D(*GP*CP*TP*TP*CP*TP*CP*TP*TP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 2.96 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-R(*GP*AP*AP*GP*AP*GP*AP*AP*GP*C)-3' Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 3.29 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 25.72Å b: 45.55Å c: 47.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.184 not available
Expression system: Not provided

Quick links

Citations

2 review citations

Recognition of the unique structure of DNA:RNA hybrids.
Shaw et al. (2008)
1 more

1 mention without citation

Directional evolution of Chlamydia trachomatis towards niche-specific adaptation.
Borges et al. (2012)