› 433d

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF A 14 BASE PAIR RNA DUPLEX WITH NONSYMMETRICAL TANDEM G.U WOBBLE BASE PAIRS

Released:
DOI: 10.2210/pdb433d/pdb
PDB entry 433d coloured by chain, front view.
PDB entry 433d coloured by chain, side view.
PDB entry 433d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Crystal structure of a 14 bp RNA duplex with non-symmetrical tandem GxU wobble base pairs.
Trikha J, Filman DJ, Hogle JM
Nucleic Acids Res 27 1728-39 (1999)
PMID: 10076005

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196767 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
5'-R(*GP*GP*UP*AP*UP*UP*GP*CP*GP*GP*UP*AP*CP*C)-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 14 nucleotides
Theoretical weight: 4.48 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P6322
Unit cell:
a: 45.4Å b: 45.4Å c: 147.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 0.277
Expression system: Not provided

Quick links

Citations

3 review citations

On the wobble GoU and related pairs.
Masquida et al. (2000)
2 more

8 mentions without citation

Major groove width variations in RNA structures determined by NMR and impact of 13C residual chemical shift anisotropy and 1H-13C residual dipolar coupling on refinement.
Tolbert et al. (2010)
7 more