› 464d

X-ray diffraction
1.23Å resolution

DISORDER AND TWIN REFINEMENT OF RNA HEPTAMER DOUBLE HELIX

Released:
DOI: 10.2210/pdb464d/pdb
PDB entry 464d coloured by chain, front view.
PDB entry 464d coloured by chain, side view.
PDB entry 464d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Disorder and twin refinement of RNA heptamer double helices.
Mueller U, Muller YA, Herbst-Irmer R, Sprinzl M, Heinemann U
Acta Crystallogr D Biol Crystallogr 55 1405-13 (1999)
PMID: 10417408

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196774 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (5'-R(*UP*AP*GP*CP*UP*CP*C)-3') Chains: A, C
Molecule details ›
Chains: A, C
Length: 7 nucleotides
Theoretical weight: 2.16 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA (5'-R(*GP*GP*GP*GP*CP*(IU)P*A)-3') Chains: B, D
Molecule details ›
Chains: B, D
Length: 7 nucleotides
Theoretical weight: 2.4 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SR 1 x SR
Ligand NA 1 x NA
1 modified residue:
Ligand IU 2 x IU

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P1
Unit cell:
a: 26.375Å b: 28.995Å c: 28.995Å
α: 110.37° β: 96.62° γ: 96.62°
R-values:
R R work R free
0.129 0.127 0.193
Expression system: Not provided

Quick links

Citations

1 review citation

Interaction of Na+, K+, Mg2+ and Ca2+ counter cations with RNA.
Kolev et al. (2018)
8 other articles

1 mention without citation

An atlas of RNA base pairs involving modified nucleobases with optimal geometries and accurate energies.
Chawla et al. (2015)