› 469d

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA

Released:
DOI: 10.2210/pdb469d/pdb
PDB entry 469d coloured by chain, front view.
PDB entry 469d coloured by chain, side view.
PDB entry 469d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA.
Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M
Nat Struct Biol 6 535-9 (1999)
PMID: 10360355

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196777 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (5'-R(*(C43)P*(G48)P*(C43)P*(G48)P*(A44)P*(A44)P*(U36)P*(U36)P*(C43)P*(G48)P*(C43)P*(G48))-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 12 nucleotides
Theoretical weight: 4.52 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
4 modified residues:
Ligand C43 8 x C43
Ligand G48 8 x G48
Ligand A44 4 x A44
Ligand U36 4 x U36

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: C2
Unit cell:
a: 41.2Å b: 34.44Å c: 46.62Å
α: 90° β: 92.4° γ: 90°
R-values:
R R work R free
0.206 0.206 0.258
Expression system: Not provided

Quick links

Citations

21 review citations

RNA targeting therapeutics: molecular mechanisms of antisense oligonucleotides as a therapeutic platform.
Bennett et al. (2010)
20 more

2 mentions without citation

Beyond ribose and phosphate: Selected nucleic acid modifications for structure-function investigations and therapeutic applications.
Liczner et al. (2021)
1 more