› 4a0c

X-ray diffraction
3.8Å resolution

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-158755 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cullin-associated NEDD8-dissociated protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1253 amino acids
Theoretical weight: 139.23 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q86VP6 (Residues: 1-1230; Coverage: 100%)
Gene names: CAND1, KIAA0829, TIP120, TIP120A
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Cullin-4B Chains: C, E
Molecule details ›
Chains: C, E
Length: 741 amino acids
Theoretical weight: 86.96 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q13620 (Residues: 192-913; Coverage: 79%)
Gene names: CUL4B, KIAA0695
Sequence domains:
E3 ubiquitin-protein ligase RBX1, N-terminally processed Chains: D, F
Molecule details ›
Chains: D, F
Length: 98 amino acids
Theoretical weight: 11.33 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62878 (Residues: 12-108; Coverage: 90%)
Gene name: Rbx1
Sequence domains: RING-H2 zinc finger domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 77.09Å b: 152.36Å c: 263.01Å
α: 90° β: 89.37° γ: 90°
R-values:
R R work R free
0.242 0.238 0.319
Expression system: Trichoplusia ni

Quick links

Citations

83 review citations

Induced protein degradation: an emerging drug discovery paradigm.
Lai et al. (2017)
82 more

7 mentions without citation

Targeted protein degradation as a powerful research tool in basic biology and drug target discovery.
Wu et al. (2020)
6 more