Function and Biology Details
Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
- not assigned
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129062 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic checkpoint serine/threonine-protein kinase BUB1
Molecule details ›
Chains: A, B, C, D
Length: 152 amino acids
Theoretical weight: 18.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Mad3/BUB1 homology region 1
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Length: 152 amino acids
Theoretical weight: 18.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
O43683 (Residues: 1-150; Coverage: 14%)
Sequence domains: Mad3/BUB1 homology region 1
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Kinetochore scaffold 1
Molecule details ›
Chains: E, F, G, H
Length: 53 amino acids
Theoretical weight: 5.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: MELT motif
Length: 53 amino acids
Theoretical weight: 5.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
- Canonical: Q8NG31 (Residues: 176-226; Coverage: 2%)
Sequence domains: MELT motif
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ESRF BEAMLINE ID14-1
Spacegroup:
P21
Expression systems:
- Escherichia coli BL21(DE3)
- Escherichia coli BL21
