› 4a2o

X-ray diffraction
1.69Å resolution

STRUCTURE OF THE HUMAN EOSINOPHIL CATIONIC PROTEIN IN COMPLEX WITH SULFATE ANIONS

Released:
DOI: 10.2210/pdb4a2o/pdb
PDB entry 4a2o coloured by chain, front view.
PDB entry 4a2o coloured by chain, side view.
PDB entry 4a2o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The sulfate-binding site structure of the human eosinophil cationic protein as revealed by a new crystal form.
Boix E, Pulido D, Moussaoui M, Nogués MV, Russi S
J Struct Biol 179 1-9 (2012)
PMID: 22579681

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146306 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eosinophil cationic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 15.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12724 (Residues: 28-160; Coverage: 100%)
Gene names: ECP, RNASE3, RNS3
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 14 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 92.264Å b: 51.29Å c: 55.394Å
α: 90° β: 111.28° γ: 90°
R-values:
R R work R free
0.165 0.162 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Nucleotide binding architecture for secreted cytotoxic endoribonucleases.
Boix et al. (2013)
3 other articles

5 mentions without citation

Human antimicrobial peptides and proteins.
Wang G. (2014)
4 more