› 4a3p

X-ray diffraction
1.4Å resolution

Structure of USP15 DUSP-UBL deletion mutant

Released:
DOI: 10.2210/pdb4a3p/pdb
PDB entry 4a3p coloured by chain, front view.
PDB entry 4a3p coloured by chain, side view.
PDB entry 4a3p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural variability of the ubiquitin specific protease DUSP-UBL double domains.
Elliott PR, Liu H, Pastok MW, Grossmann GJ, Rigden DJ, Clague MJ, Urbé S, Barsukov IL
FEBS Lett 585 3385-90 (2011)
PMID: 22001210

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195233 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 15 Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 25.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9Y4E8 (Residues: 6-223; Coverage: 22%)
Gene names: KIAA0529, USP15
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand IOD 1 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 44.95Å b: 44.25Å c: 56.15Å
α: 90° β: 104.33° γ: 90°
R-values:
R R work R free
0.157 0.153 0.221
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

The role of UBL domains in ubiquitin-specific proteases.
Faesen et al. (2012)
5 more

2 mentions without citation

Structural Basis of the Recruitment of Ubiquitin-specific Protease USP15 by Spliceosome Recycling Factor SART3.
Zhang et al. (2016)
1 more