› 4a4d

X-ray diffraction
2.7Å resolution

Crystal structure of the N-terminal domain of the Human DEAD-BOX RNA helicase DDX5 (P68)

Released:
DOI: 10.2210/pdb4a4d/pdb
PDB entry 4a4d coloured by chain, front view.
PDB entry 4a4d coloured by chain, side view.
PDB entry 4a4d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The variable N-terminal region of DDX5 contains structural elements and auto-inhibits its interaction with NS5B of hepatitis C virus.
Dutta S, Gupta G, Choi YW, Kotaka M, Fielding BC, Song J, Tan YJ
Biochem J 446 37-46 (2012)
PMID: 22640416

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148187 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable ATP-dependent RNA helicase DDX5 Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P17844 (Residues: 52-304; Coverage: 41%)
Gene names: DDX5, G17P1, HELR, HLR1
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: I222
Unit cell:
a: 66.18Å b: 73.801Å c: 104Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.262 0.293
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

DDX5 RNA Helicases: Emerging Roles in Viral Infection.
Cheng et al. (2018)
3 more

8 mentions without citation

P68 RNA helicase as a molecular target for cancer therapy.
Dai et al. (2014)
7 more