› 4a51

X-ray diffraction
2.75Å resolution

Crystal structure of human kinesin Eg5 in complex with 1-(3-(((2-Aminoethyl)thio)diphenylmethyl)phenyl)ethanone hydrochloride

Released:
DOI: 10.2210/pdb4a51/pdb
PDB entry 4a51 coloured by chain, front view.
PDB entry 4a51 coloured by chain, side view.
PDB entry 4a51 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Triphenylbutanamines: kinesin spindle protein inhibitors with in vivo antitumor activity.
Wang F, Good JA, Rath O, Kaan HY, Sutcliffe OB, Mackay SP, Kozielski F
J Med Chem 55 1511-25 (2012)
PMID: 22248262

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF11 Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 368 amino acids
Theoretical weight: 41.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52732 (Residues: 1-368; Coverage: 35%)
Gene names: EG5, KIF11, KNSL1, TRIP5
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

5 bound ligands:
Ligand ADP 7 x ADP
Ligand MG 7 x MG
Ligand DQ8 7 x DQ8
Ligand CL 14 x CL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 145.84Å b: 156.4Å c: 170.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.221 0.28
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Kinesins and cancer.
Rath et al. (2012)
3 more

5 mentions without citation

Focus on Marine Animal Safety and Marine Bioresources in Response to the SARS-CoV-2 Crisis.
Yang et al. (2022)
4 more