4adf

X-ray diffraction
4.4Å resolution

CRYSTAL STRUCTURE OF THE HUMAN COLONY-STIMULATING FACTOR 1 (hCSF-1) CYTOKINE IN COMPLEX WITH THE VIRAL RECEPTOR BARF1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-136666 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Secreted protein BARF1 Chains: A, B, C, D, E, F, M, N, O, P, Q, R
Molecule details ›
Chains: A, B, C, D, E, F, M, N, O, P, Q, R
Length: 208 amino acids
Theoretical weight: 23.41 KDa
Source organism: human gammaherpesvirus 4
Expression system: Homo sapiens
UniProt:
  • Canonical: P03228 (Residues: 21-221; Coverage: 100%)
Gene name: BARF1
Macrophage colony-stimulating factor 1 43 kDa subunit Chains: G, H, I, J, K, L, S, T, U, V, W, X
Molecule details ›
Chains: G, H, I, J, K, L, S, T, U, V, W, X
Length: 153 amino acids
Theoretical weight: 17.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09603 (Residues: 33-181; Coverage: 29%)
Gene name: CSF1
Sequence domains: Macrophage colony stimulating factor-1 (CSF-1)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3121
Unit cell:
a: 218.44Å b: 218.44Å c: 331.17Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.238 0.276
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)