› 4ae4

X-ray diffraction
1.65Å resolution

The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a novel SOUBA domain

Released:
DOI: 10.2210/pdb4ae4/pdb
PDB entry 4ae4 coloured by chain, front view.
PDB entry 4ae4 coloured by chain, side view.
PDB entry 4ae4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.
Agromayor M, Soler N, Caballe A, Kueck T, Freund SM, Allen MD, Bycroft M, Perisic O, Ye Y, McDonald B, Scheel H, Hofmann K, Neil SJ, Martin-Serrano J, Williams RL
Structure 20 414-28 (2012)
PMID: 22405001

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192730 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 118 amino acids
Theoretical weight: 13.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZ09 (Residues: 389-502; Coverage: 23%)
Gene names: NAG20, UBAP1
Sequence domains: Ubiquitin-associated protein 1, UBA2 domain
Structure domains: UBAP1 SOUBA domain
Ubiquitin-associated protein 1 Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 13.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZ09 (Residues: 389-502; Coverage: 23%)
Gene names: NAG20, UBAP1
Sequence domains: Ubiquitin-associated protein 1, UBA2 domain
Structure domains: UBAP1 SOUBA domain

Ligands and Environments

4 bound ligands:
Ligand NHE 2 x NHE
Ligand K 4 x K
Ligand GOL 1 x GOL
Ligand NA 1 x NA
2 modified residues:
Ligand MSE 16 x MSE
Ligand MSO 2 x MSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P1
Unit cell:
a: 34.404Å b: 43.48Å c: 59.413Å
α: 102.58° β: 96.47° γ: 113.24°
R-values:
R R work R free
0.152 0.149 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

The many functions of ESCRTs.
Vietri et al. (2020)
22 more

3 mentions without citation

Membrane fission reactions of the mammalian ESCRT pathway.
McCullough et al. (2013)
2 more