› 4ae8

X-ray diffraction
1.59Å resolution

Crystal structure of human THEM4

Released:
DOI: 10.2210/pdb4ae8/pdb
PDB entry 4ae8 coloured by chain, front view.
PDB entry 4ae8 coloured by chain, side view.
PDB entry 4ae8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Acyl coenzyme A thioesterase Them5/Acot15 is involved in cardiolipin remodeling and fatty liver development.
Zhuravleva E, Gut H, Hynx D, Marcellin D, Bleck CK, Genoud C, Cron P, Keusch JJ, Dummler B, Esposti MD, Hemmings BA
Mol Cell Biol 32 2685-97 (2012)
PMID: 22586271

Function and Biology Details

Reaction catalysed: 
Palmitoyl-CoA + H(2)O = CoA + palmitate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl-coenzyme A thioesterase THEM4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 211 amino acids
Theoretical weight: 23.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5T1C6 (Residues: 37-240; Coverage: 85%)
Gene names: CTMP, THEM4
Sequence domains: Thioesterase superfamily
Structure domains: Hotdog Thioesterase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 50.539Å b: 58.211Å c: 69.681Å
α: 89.96° β: 71.14° γ: 64.6°
R-values:
R R work R free
0.187 0.185 0.223
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Formation and regulation of mitochondrial membranes.
Schenkel et al. (2014)
5 more

3 mentions without citation

Acyl coenzyme A thioesterase Them5/Acot15 is involved in cardiolipin remodeling and fatty liver development.
Zhuravleva et al. (2012)
2 more