› 4ajp

X-ray diffraction
2.38Å resolution

Human LDHA in complex with 2-((4-(4-((3-((2-methyl-1,3-benzothiazol- 6yl)amino)-3-oxo-propyl)amino)-4-oxo-butyl)phenyl)methyl)propanedioic acid

Released:
DOI: 10.2210/pdb4ajp/pdb
PDB entry 4ajp coloured by chain, front view.
PDB entry 4ajp coloured by chain, side view.
PDB entry 4ajp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Design and synthesis of novel lactate dehydrogenase A inhibitors by fragment-based lead generation.
Ward RA, Brassington C, Breeze AL, Caputo A, Critchlow S, Davies G, Goodwin L, Hassall G, Greenwood R, Holdgate GA, Mrosek M, Norman RA, Pearson S, Tart J, Tucker JA, Vogtherr M, Whittaker D, Wingfield J, Winter J, Hudson K
J Med Chem 55 3285-306 (2012)
PMID: 22417091

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase A chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 337 amino acids
Theoretical weight: 37.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00338 (Residues: 2-332; Coverage: 100%)
Gene names: LDHA, PIG19
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 88N 4 x 88N
Ligand SO4 11 x SO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 63.98Å b: 136.78Å c: 83.79Å
α: 90° β: 102.31° γ: 90°
R-values:
R R work R free
0.168 0.166 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Twenty years on: the impact of fragments on drug discovery.
Erlanson et al. (2016)
18 more

6 mentions without citation

Three-Dimensional Analysis of the Interactions between hLDH5 and Its Inhibitors.
Poli et al. (2017)
5 more