› 4ajx

X-ray diffraction
1.2Å resolution

Ligand controlled assembly of hexamers, dihexamers, and linear multihexamer structures by an engineered acylated insulin

Released:
DOI: 10.2210/pdb4ajx/pdb
PDB entry 4ajx coloured by chain, front view.
PDB entry 4ajx coloured by chain, side view.
PDB entry 4ajx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ligand-controlled assembly of hexamers, dihexamers, and linear multihexamer structures by the engineered acylated insulin degludec.
Steensgaard DB, Schluckebier G, Strauss HM, Norrman M, Thomsen JK, Friderichsen AV, Havelund S, Jonassen I
Biochemistry 52 295-309 (2013)
PMID: 23256685

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134235 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 29 amino acids
Theoretical weight: 3.33 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-53; Coverage: 34%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

5 bound ligands:
Ligand 16E 2 x 16E
Ligand RCO 6 x RCO
Ligand NA 2 x NA
Ligand IMD 3 x IMD
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 47.31Å b: 62.49Å c: 57.27Å
α: 90° β: 111.8° γ: 90°
R-values:
R R work R free
0.142 0.14 0.16
Expression system: Saccharomyces cerevisiae

Quick links

Citations

16 review citations

A review of the pharmacological properties of insulin degludec and their clinical relevance.
Haahr et al. (2014)
15 more

4 mentions without citation

How Structural Biologists and the Protein Data Bank Contributed to Recent FDA New Drug Approvals.
Westbrook et al. (2019)
3 more