› 4ap0

X-ray diffraction
2.59Å resolution

The mitotic kinesin Eg5 in complex with Mg-ADP and ispinesib

Released:
DOI: 10.2210/pdb4ap0/pdb
PDB entry 4ap0 coloured by chain, front view.
PDB entry 4ap0 coloured by chain, side view.
PDB entry 4ap0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the ternary Eg5-ADP-ispinesib complex.
Talapatra SK, Schüttelkopf AW, Kozielski F
Acta Crystallogr D Biol Crystallogr 68 1311-9 (2012)
PMID: 22993085

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF11 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 370 amino acids
Theoretical weight: 41.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52732 (Residues: 1-368; Coverage: 35%)
Gene names: EG5, KIF11, KNSL1, TRIP5
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand ADP 4 x ADP
Ligand CL 6 x CL
Ligand G7X 4 x G7X
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 64.723Å b: 112.607Å c: 106.913Å
α: 90° β: 90.02° γ: 90°
R-values:
R R work R free
0.206 0.204 0.252
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Kinesin spindle protein (KSP) inhibitors in combination with chemotherapeutic agents for cancer therapy.
Song et al. (2013)
2 more

16 mentions without citation

Force generation by kinesin and myosin cytoskeletal motor proteins.
Kull et al. (2013)
15 more